Article Dans Une Revue Spectrochimica Acta Part B: Atomic Spectroscopy Année : 2025

A multi-technique platform for the quantification and identification of selenoneine with a focus on HPLC-HG-AFS

Résumé

Selenoneine, a naturally occurring form of selenium (Se) in biota, has recently raised considerable interest in the fields of nutrition, drug development and environmental research. Selenoneine has a strong antioxidant capacity which makes it a potential Se supplement for humans or farm animals. There is a lack of a reliable and cost-effective online speciation methodology for the determination of such a biologically relevant Se species. In this study, a method has been developed for the simultaneous analysis of selenoneine and other organic Se species. It is based on the online coupling of reversed-phase liquid chromatography, ultraviolet radiation, thermal treatment, hydride generation and atomic fluorescence spectrometry (HPLC-UV-TT-HG-AFS). Quantitative determination of selenoneine by external calibration and its separation from commonly found organic Se species (selenomethionine, Se-methyl-selenocysteine) was achieved. The proposed methodology resulted in a linear response (R2 > 0.999) for a concentration range of 5–100 μgSe L−1 and a limit of detection of 0.5 μgSe L−1. The repeatability was 0.8 %, with a reproducibility of 1.6 %. This analytical approach is versatile, cost-effective, and fast with a chromatographic runtime of less than 12 min. The high selectivity of HPLC-ICP-MS, combined with the species identification power of HPLC-ESI-MS/MS was found invaluable for gaining further speciation insights and confirming the results obtained by HPLC-UV-TT-HG-AFS. The developed approach could serve as useful tool for routine selenoneine analysis in biological samples as shown here with the analysis of seabird liver extracts.

Fichier sous embargo
Fichier sous embargo
0 0 8
Année Mois Jours
Avant la publication
mardi 12 mai 2026
Fichier sous embargo
mardi 12 mai 2026
Connectez-vous pour demander l'accès au fichier

Dates et versions

hal-05172037 , version 1 (12-11-2025)

Licence

Identifiants

Citer

Claudia Marchán-Moreno, Christian Ward-Deitrich, Jasmina Allen, Maïté Bueno, David Amouroux, et al.. A multi-technique platform for the quantification and identification of selenoneine with a focus on HPLC-HG-AFS. Spectrochimica Acta Part B: Atomic Spectroscopy, 2025, 232, pp.107261. ⟨10.1016/j.sab.2025.107261⟩. ⟨hal-05172037⟩
111 Consultations
8 Téléchargements

Altmetric

Partager

  • More