Kinetics of meta-metabolome response to cobalt exposure in dynamic river biofilm communities - Université de Pau et des Pays de l'Adour Accéder directement au contenu
Poster De Conférence Année : 2024

Kinetics of meta-metabolome response to cobalt exposure in dynamic river biofilm communities

Soizic Morin
Clémentine Gelber
  • Fonction : Auteur
Patrick Baldoni-Andrey
Claude Fortin

Résumé

As part of the energy transition away from fossil fuels, the extraction and use of cobalt (Co) has increased significantly in recent decades. Although few studies have investigated its toxicity, it is currently classified as a substance to monitor in rivers in some countries. To provide Co toxicity data needed to assess the ecological risk for aquatic organisms, mature biofilms were exposed for one month in artificial river streams to four Co concentrations (background concentration, 1 x 10-7, 5 x 10-7 and 1 x 10-6 M). Bioaccumulation, biomass, chlorophyll-a levels and the composition and structure of prokaryotic and eukaryotic communities were monitored along with the meta-metabolomic response at different time points (from 1 hour to 28 days). Using an untargeted metabolomic approach, the entire biofilm meta-metabolome was characterized using a new holistic data processing approach based on dose-response modeling, benchmark-dose/time calculation and characterization of response trends. From these results, two ranges of concentrations were observed at each time step. The first range was characterized as a concentration range inducing defense responses (CRIDeR) and the second as a concentration range inducing damage responses (CRIDaR). The initial thresholds of CRIDeR were found to be similar between each exposure time, whereas those of CRIDaR increased as a function of exposure time. This result suggested a community shift toward tolerant species, which was confirmed with the characterization of the prokaryotic and eukaryotic communities. Moreover, we also demonstrated that changes in the meta-metabolome were faster than changes in community composition using a Multivariate Omics Trajectory Analysis. These rapid changes in the meta-metabolome were confirmed by the observation of time ranges inducing defense and damage responses (TRIDeR and TRIDaR) based also on the interpretation of trends in dose-response models of untargeted meta-metabolomic features as a function of exposure time. The initial thresholds of TRIDeR were measured to be a few seconds whereas those of TRIDaR were in the order of 1 day. This study demonstrates that meta-metabolome defenses and damage responses of Co-exposed biofilms are rapidly and continuously impaired, even within resistant and tolerant microbial communities.
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Dates et versions

hal-04591639 , version 1 (29-05-2024)

Identifiants

  • HAL Id : hal-04591639 , version 1

Citer

Simon Colas, Benjamin Marie, Soizic Morin, Mathieu Milhe-Poutingon, Pierre Foucault, et al.. Kinetics of meta-metabolome response to cobalt exposure in dynamic river biofilm communities. SETAC Europe 34th Annual Meeting, May 2024, Seville, Spain. ⟨hal-04591639⟩
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