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Poster De Conférence Année : 2024

The use of concentration ranges inducing defense and damage responses as a promising approach to overcome database gaps in metabolite annotation in ecotoxicology

Résumé

The use of untargeted metabolomic data by liquid chromatography-high resolution mass spectrometry (LC-HRMS) enables the characterization of families and functions of molecules within biological systems without making an a priori decision on the molecules to be identified. However, this approach has some limitations. Indeed, the proportion of annotation of untargeted metabolomic features with the use of databases proved to be very low (~5% of the chemical signal). The trends in the doseresponse model of the untargeted metabolomic feature are a biological parameter that can reflect the metabolome responses of a biological system exposed to a contaminant without the need for an annotation step. Following a meta-analysis of response trends (i.e. biphasic: bell- and U-shaped, monotonic: decreasing and increasing) of known biomarkers of defense and damage that we performed on 156 selected articles, 2,595 dose-response curves were interpreted from exposure experiments to inorganic and organic contaminants on 18 phyla, representing four kingdoms. The results of multinomial logistic models showed that defense biomarkers mainly describe biphasic trends, while damage biomarkers follow monotonic trends. Based on these observations, we have characterized the metabolomic response of river biofilms exposed to cobalt, using dose-response modeling, benchmark-dose calculations and characterization of response trends. The trends in the dose-response curves obtained for all untargeted metabolomic features revealed a concentration range in which the biofilms were likely to exhibit a defense response (predominance of untargeted metabolomic features with a biphasic trend) and a range in which the biofilms would instead exhibit a damage response (predominance of untargeted metabolomic features with a monotonic trend). Following these observations, we proposed a new concept of concentration range inducing defense responses (CRIDeR) and concentration range inducing damage responses (CRIDaR). This holistic approach makes it possible to use the entire chemical signal of the metabolome without being restricted by the metabolite annotation step. It can also be used to monitor the effects of exposure time with the characterization of a time range inducing defense responses (TRIDeR) and a time range inducing damage responses (TRIDaR). This approach represents a promising avenue for the development of a robust and sensitive tool for environmental risk assessment.
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Dates et versions

hal-04591630 , version 1 (29-05-2024)

Identifiants

  • HAL Id : hal-04591630 , version 1

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Simon Colas, Benjamin Marie, Claude Fortin, Séverine Le Faucheur. The use of concentration ranges inducing defense and damage responses as a promising approach to overcome database gaps in metabolite annotation in ecotoxicology. SETAC Europe 34th Annual Meeting, May 2024, Seville, Spain. ⟨hal-04591630⟩
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