Meta-metabolomic responses of river biofilms to cobalt exposure and use of dose-response model trends as an indicator of effects - Université de Pau et des Pays de l'Adour Accéder directement au contenu
Article Dans Une Revue Journal of Hazardous Materials Année : 2024

Meta-metabolomic responses of river biofilms to cobalt exposure and use of dose-response model trends as an indicator of effects

Résumé

The response of the meta-metabolome is rarely used to characterize the effects of contaminants on a whole community. Here, the meta-metabolomic fingerprints of biofilms were examined after 1, 3 and 7 days of exposure to five concentrations of cobalt (from background concentration to 1 × 10−5 M) in aquatic microcosms. The untargeted metabolomic data were processed using the DRomics tool to build dose-response models and to calculate benchmark-doses. This approach made it possible to use 100% of the chemical signal instead of being limited to the very few annotated metabolites (7%). These benchmark-doses were further aggregated into an empirical cumulative density function. A trend analysis of the untargeted meta-metabolomic feature dose-response curves after 7 days of exposure suggested the presence of a concentration range inducing defense responses between 1.7 × 10−9 and 2.7 × 10−6 M, and of a concentration range inducing damage responses from 2.7 × 10−6 M and above. This distinction was in good agreement with changes in the other biological parameters studied (biomass and chlorophyll content). This study demonstrated that the molecular defense and damage responses can be related to contaminant concentrations and represents a promising approach for environmental risk assessment of metals.
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Dates et versions

hal-04536517 , version 1 (08-04-2024)

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Simon Colas, Benjamin Marie, Mathieu Milhe-Poutingon, Marie-Claire Lot, Amiel Boullemant, et al.. Meta-metabolomic responses of river biofilms to cobalt exposure and use of dose-response model trends as an indicator of effects. Journal of Hazardous Materials, 2024, 470, pp.134099. ⟨10.1016/j.jhazmat.2024.134099⟩. ⟨hal-04536517⟩
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