A multi-technique analysis of gelatin biodegradation on core-shell nanoparticles surface by Alteromonas macleodii extracellular proteases
Résumé
The study of interaction mechanisms at nanoscale between nanomaterials and microbial cells is of great importance in a
wide range of research fields from health to environment. In the latter case, due to the high occurrence of nanoparticulate
systems in the environment, the in-depth investigation of nanoparticles/bacteria interactions at nanoscale is needed to
better understand the full picture of natural, chemical and biological processes of the microbial biodegradation of natural
or manufactured organic nanoparticles in ecosystems. In this paper, we highlight for the first time the proof of the
biodegradation process of gelatin adsorbed on nanoparticles surface by Alteromonas macleodii extracellular proteases.
Using model core-shell gold@silica@gelatin nanoparticles, the biodegradation process has been demonstrated through a
novel combined multi-technique approach using mass spectrometry (ToF-SIMS, LC-ESI-MS/MS, SP-ICP-MS), photo-electron
spectroscopy (XPS) and electron microscopy (SEM, TEM).
Origine | Fichiers produits par l'(les) auteur(s) |
---|