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Article Dans Une Revue Talanta Année : 2024

Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry

Mathieu Melczer
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Ana Justo-Vega
Ogier Hanser
Sophie Ndaw

Résumé

A method for the analysis of essential metals (Fe, Cu, Mg, and Zn) and non-metals (P, S) in single red blood cells was developed by single cell (SC)-ICP-MS. The use of a triple quadrupole configuration (MS/MS) enabled an effective elimination of polyatomic interferences, which affect the accuracy of ICP-MS analysis using a single quadrupole mass analyzer. Fixation with glutaraldehyde for at least 90 days was developed to improve the quantification of elements in a single red blood cell. The experimental conditions were optimized while special attention was paid to the residence time of analytes in the plasma. Addition of a surfactant (0.05% (v/v) Tween80®) improved quantification of elements in fixed red blood cells. The detection limits obtained by SC-ICP-MS/MS were lower than for ICP-MS, especially for S and P (3 fg and 1.7 fg. cell-1 instead of 163 and 6.3 fg. cell-1, respectively)
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Dates et versions

hal-04224687 , version 1 (02-10-2023)

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Mathieu Melczer, Javier Jiménez Lamana, Ana Justo-Vega, Ogier Hanser, Sophie Ndaw, et al.. Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry. Talanta, 2024, 267, pp.125226. ⟨10.1016/j.talanta.2023.125226⟩. ⟨hal-04224687⟩
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