An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla) - Université de Pau et des Pays de l'Adour
Article Dans Une Revue Journal of Steroid Biochemistry and Molecular Biology Année : 2025

An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla)

Résumé

The European eel (Anguilla anguilla) juvenile stage exhibits facultative estuarine migration. The causes of this behavior are yet unknown but it may have an impact on the population's fate by altering the sex ratio of the population. Recent studies have highlighted potential stress-related issues in glass eels settling in estuaries but studying stress response in small organisms requires sensitive, accurate and precise analytical methods. The aims of the present study are (i) to develop a whole-body Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method for the simultaneous determination of several stress hormones in low-body mass fish; (ii) to apply this method to glass eels to study their responses to acute stress (iii) to test the effect of anxiolytics (diazepam) on these responses. Our results showed that enhanced LC-MS/MS analysis reduced detection limits and improved accuracy and precision for the quantification at the individual level. Following an acute stress, cortisol concentration significantly increased in glass eels and a 15 h diazepam exposure significantly reduced cortisol levels highlighting a marked anxiolytic effect on this species.
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Dates et versions

hal-04776872 , version 1 (12-11-2024)

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Stellia Sebihi, Mathilde Monperrus, Pascale Coste-Heinrich, Emmanuel Huchet, Matthieu Lingrand, et al.. An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla). Journal of Steroid Biochemistry and Molecular Biology, 2025, 245, pp.106627. ⟨10.1016/j.jsbmb.2024.106627⟩. ⟨hal-04776872⟩
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