Extraction recovery and speciation of selenium in Se-enriched yeast - Université de Pau et des Pays de l'Adour
Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2024

Extraction recovery and speciation of selenium in Se-enriched yeast

Résumé

The complete characterization of selenium-enriched yeast in terms of selenium species has been the goal of extensive research for the last three decades. This contribution addresses the two outstanding questions: the mass balance of the identified and reported selenium species and the possible presence of inorganic selenium. For this purpose, four procedures have been designed combining, in diverse order, the principal steps of selenium speciation analysis in Se-rich yeast: extraction of the Se-metabolome, derivatization of cysteine and Se-cysteine (SeCys) residues, proteolysis, and definitive Se recovery using SDS extraction, followed by mineralization. The recovery of selenium in each step and its speciation were controlled by ICP MS and by reversed-phase HPLC-ICP MS, respectively. The study, carried out for the SELM-1 reference material, demonstrated the presence of about 10% of inorganic selenium and a serious risk of losses of SeCys during derivatization and proteolysis. As result of our work, we postulate the following values for SELM-1: Se-metabolome fraction (SeMF) 14.8 ± 0.7%; total selenomethionine (SeMet) 66.2 ± 2.7% (including ca. 1.5% SeMet present in the SeMF); total SeCys 12.5 ± 1.5% (including 2% of SeCys present in the Se-MF); total inorganic selenium 9.7 ± 1.7%, accounting for > 99.8% of the selenium.
Fichier non déposé

Dates et versions

hal-04672949 , version 1 (19-08-2024)

Identifiants

Citer

Ange Angaïts, Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski. Extraction recovery and speciation of selenium in Se-enriched yeast. Analytical and Bioanalytical Chemistry, 2024, 416, pp.5111-5120. ⟨10.1007/s00216-024-05448-w⟩. ⟨hal-04672949⟩
20 Consultations
0 Téléchargements

Altmetric

Partager

More