Culturing the desert microbiota - Université de Pau et des Pays de l'Adour Access content directly
Journal Articles Frontiers in Microbiology Year : 2023

Culturing the desert microbiota


Over the last 30 years, the description of microbial diversity has been mainly based on culture-independent approaches (metabarcoding and metagenomics) allowing an in-depth analysis of microbial diversity that no other approach allows. Bearing in mind that culture-dependent approaches cannot replace culture-independent approaches, we have improved an original method for isolating strains consisting of "culturing" grains of sand directly on Petri dishes (grain-by-grain method). This method allowed to cultivate up to 10% of the bacteria counted on the surface of grains of the three sites studied in the Great Western Erg in Algeria (Timoudi, Béni Abbès, and Taghit), knowing that on average about 10 bacterial cells colonize each grain. The diversity of culturable bacteria (collection of 290 strains) predicted by 16S rRNA gene sequencing revealed that Arthrobacter subterraneus, Arthrobacter tecti, Pseudarthrobacter phenanthrenivorans, Pseudarthrobacter psychrotolerans, and Massilia agri are the dominant species. The comparison of the culture-dependent andindependent (16S rRNA gene metabarcoding) approaches at the Timoudi site revealed 18 bacterial genera common to both approaches with a relative overestimation of the genera Arthrobacter/Pseudarthrobacter and Kocuria, and a relative underestimation of the genera Blastococcus and Domibacillus by the bacterial culturing approach. The bacterial isolates will allow further study on the mechanisms of tolerance to desiccation, especially in Pseudomonadota (Proteobacteria).
Fichier principal
Vignette du fichier
fmicb-14-1098150.pdf (2.67 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-04094404 , version 1 (11-05-2023)



Zakia Selmani, Eléonore Attard, Béatrice Lauga, Mohamed Barakat, Philippe Ortet, et al.. Culturing the desert microbiota. Frontiers in Microbiology, 2023, 14, pp.1098150. ⟨10.3389/fmicb.2023.1098150⟩. ⟨hal-04094404⟩
73 View
17 Download



Gmail Facebook X LinkedIn More