Glyphosate–Exonuclease Interactions: Reduced Enzymatic Activity as a Route to Glyphosate Biosensing - Université de Pau et des Pays de l'Adour Access content directly
Journal Articles Macromolecular Bioscience Year : 2023

Glyphosate–Exonuclease Interactions: Reduced Enzymatic Activity as a Route to Glyphosate Biosensing

Abstract

N-phosphonomethyle-glycine (glyphosate) is the most widely used pesticideworldwide due to its effectiveness in killing weeds at a moderate cost,bringing significant economic benefits. However, owing to its massive use,glyphosate and its residues contaminate surface waters. On site, fastmonitoring of contamination is therefore urgently needed to alert localauthorities and raise population awareness. Here the hindrance of the activityof two enzymes, the exonuclease I (Exo I) and the T5 exonuclease (T5 Exo) byglyphosate, is reported. These two enzymes digest oligonucleotides intoshorter sequences, down to single nucleotides. The presence of glyphosate inthe reaction medium hampers the activity of both enzymes, slowing downenzymatic digestion. It is shown by fluorescence spectroscopy that theinhibition of ExoI enzymatic activity is specific to glyphosate, paving the wayfor the development of a biosensor to detect this pollutant in drinking water atsuitable detection limits, i.e., 0.6 nm
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Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

hal-04055735 , version 1 (04-04-2023)

Identifiers

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Mohamed Amine Berkal, Quentin Palas, Estelle Ricard, Christine Lartigau-Dagron, Luisa Ronga, et al.. Glyphosate–Exonuclease Interactions: Reduced Enzymatic Activity as a Route to Glyphosate Biosensing. Macromolecular Bioscience, 2023, 23 (8), pp.2200508. ⟨10.1002/mabi.202200508⟩. ⟨hal-04055735⟩
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