%0 Journal Article %T Extraction of Mycosporine-like Amino Acids and Proteins from the Agarophyte Gelidium corneum Using Pulsed Power Techniques %+ Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) %+ Laboratoire des Sciences de l'Ingénieur Appliquées à la Mécanique et au génie Electrique (SIAME) %+ Transformation Intégrée de la Matière Renouvelable (TIMR) %+ Universidade de Aveiro %+ Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (UPV/EHU) %+ KTH Royal Institute of Technology [Stockholm] (KTH ) %A Mcreynolds, Colin %A Adrien, Amandine %A de Ferron, Antoine Silvestre %A Pecastaing, Laurent %A Boussetta, Nadia %A Grimi, Nabil %A Fernandes, Susana %@ 2304-8158 %J Foods %I MDPI %V 12 %N 7 %P 1473 %8 2023-04 %D 2023 %R 10.3390/foods12071473 %K red algae; seaweeds; mycosporine-like amino acids; proteins; marine bioresources; valorization; sustainable extractions; high-voltage electrical discharges; pulsed electrical fields %Z Environmental Sciences %Z Chemical Sciences %Z Chemical Sciences/PolymersJournal articles %X Gelidium corneum (syn. sesquipedale) is an industrially and ecologically important species of red alga used for the production of high-quality agar. However, the species is also of growing interest for the production of other valuable compounds, such as mycosporine-like amino acids (MAAs), with potential cosmeceutical and biomedical applications. Novel methods using two pulsed power techniques, high-voltage electrical discharges (HVED) and pulsed electrical fields (PEF), were evaluated for efficacy of MAA extraction. Algal suspensions were prepared at two ratios (1:20 and 1:40 w:v). Four different extraction protocols were compared: (i) high-voltage electrical discharges, (ii) pulsed electric fields, (iii) maceration at room temperature, and (iv) maceration at 50 °C. The algae were treated in three states: freshly harvested, dried, and powdered. HVED and PEF treatments were effective when performed on fresh algae, and in particular the HVED treatment resulted in yields of MAAs twenty times higher than the control: 0.81 ± 0.05 mg/gDry Weight (DW) vs. 0.037 ± 0.002 mg/gDW. This effect was not observed to the same extent when the algae were dried or powdered, although HVED remained the most selective method overall. %G English %2 https://univ-pau.hal.science/hal-04067037/document %2 https://univ-pau.hal.science/hal-04067037/file/foods-12-01473.pdf %L hal-04067037 %U https://univ-pau.hal.science/hal-04067037 %~ SDE %~ CNRS %~ UNIV-COMPIEGNE %~ UNIV-PAU %~ GIP-BE %~ IPREM %~ INC-CNRS %~ IPREM-PCM %~ TIMR %~ SIAME %~ ANR %~ UPPA-OA %~ ALLIANCE-SU